INTRODUCTION
Heterocyclic compounds, organic compounds with at least one carbon atom linked to a different atom (typically oxygen, nitrogen, or sulfur), have been extensively examined due to the abundance and variety of their natural or industrially synthesized derivatives [1, 2]. Among these, Schiff bases (or imines or azomethines), which are nitrogen analogs of aldehydes or ketones where the carbonyl group (CO) is substituted by an imine or azomethine group, exhibit various pharmacological activities such as antimicrobial, antitubercular, antiviral, and antimalarial [3-7]. Current research focuses on the development of anticancer drugs with unique structural properties that display distinct cytotoxicity while avoiding systemic toxic drug effects [8-10]. Thiazole, a 5-membered heterocyclic motif containing sulfur and nitrogen atoms, is of specific interest due to its key role in many medicinally important compounds [11]. Its derivatives have received significant attention for their varied applications as analytical reagents, antioxidants, antifungals, and anticancer agents [12, 13]. They also exhibit diverse pharmacological activities, such as acting as antidepressants [14-18]. The current study examines the synthesis of a novel compound (DEAP) using an array of spectral methods (1H,13C-NMR, Mass, UV-Vis, Infrared FT-IR, XRD, FE-SEM, and C.H.N.S) and assesses its biological activities. In particular, the study evaluates the compound’s potential as an anticancer drug, using prostate and breast cancer (MCF-7) cell lines, and contrasts its effects with those on normal cell lines using the MTT assay. 

MATERIALS AND METHODS
Chemicals and materials
The subsequent chemicals were obtained commercially and utilized without additional purification: 2-aminothiazol (C3H4N2S), 4-aminoacetophenone (C8H9NO), benzil (C14H10O2), and 2-aminophenol (C6H7NO), sourced from Sigma-Aldrich (Germany). Ethanol Absolute (CH3CH2OH) was procured from Scharlau. All reagents employed were of analytical grade purity, requiring no further purification, and all solutions were prepared using deionized water.

Instruments
The chemical (DEAP) was elementally microanalyzed utilizing an EA 300 (C.H.N.S) element analyzer. DMSO-d6 was the solvent and TMS was the internal reference for 1H and 13C NMR spectra on a Bruker 400 MHZ spectrometer. A Shimadzu Agilent Technologies 5973C at 70 eV produced mass spectra. Using 100% ethanol in a 1 cm quartz cuvette, a T80-PG double beam (UV-Vis) spectrophotometer measured electronic spectra from 200 to 800 nm. A Shimadzu 8400 S captured KBr disk FT-IR spectra (4000400 cm-1). MIRA3 TESCANs captured FESEM images. Bestec Aluminium anode-Germany XRD tests were performed. X-ray diffractometer utilizing (Cu Kα) radiation (1.41.5418 A°) from (5-80°).

Preparation of (DEAP)
The synthesis of compound (DEAP) was accomplished in two stages (Fig. 1). The first stage entailed the creation of compound A, (E)-4-(1-(thiazo-2-ylimino) ethyl) aniline. This was prepared by dissolving (1.5 g, 1 mmol.) of 2-aminothiazol in (25 ml) of absolute ethanol, followed by the addition of (1.35 g, 1 mmol.) of 4-aminoacetophenone, also dissolved in (25 ml) of absolute ethanol, while stirring continuously. Post-refluxing the mixture for 8 hours, it was cooled, and a precipitate was observed. The precipitate was filtered, dried, and recrystallized from absolute ethanol, yielding a 73% product with a melting point of 207°C. The second stage involved creating compound DEAP by dissolving compound A (2.1 g, 1 mmol.) in (15 ml) of absolute ethanol. To this, a solution of benzil (2.1 g, 1 mmol.) and 2-aminophenol (1g, 1 mmol.), each dissolved in absolute ethanol (25 ml and 15 ml, respectively), was added while continuously stirring. Additionally, 4-5 drops of glacial acetic acid were introduced into the mixture. After refluxing this mixture for 8 hours and subsequently cooling it, a precipitate formed. This was filtered, dried, and recrystallized from absolute ethanol, leading to the formation of compound (DEAP) 2-(((1E,2E)-1,2-diphenyl-2-((4(E-1-(thiazol-2-ylimino) ethyl) phenyl) imino) ethylidene) amino) phenol as a solid brown product with an 87% yield and a melting point of 227°C. Elemental analysis was consistent with the formula for compound (DEAP) presented in Table 1.

RESULTS AND DISCUSSION
The functional groups have been confirmed via Fourier-transform infrared spectroscopy (FTIR) analysis[19], Fig. 2a: showed the band at 3329cm-1 for (OH) phenol, 2926 cm-1 for (C-H) Aromatic, 2844 cm-1 for (C-H) Aliphatic , 1529 cm-1 for (C=N) thiazole ring, 1643 cm-1for (C=N) imine , 1443 cm-1 for (C=C) Aromatic, 1279 cm-1 for (C-S). UV–visible absorption spectrum was obtained at room temperature for the compound (DEAP), in Fig. 2b includes three absorption peaks, two of which are two peaks at 205 nm (148780 cm-1) and 244 nm (40984cm-1) which are due to the π-π* type transition resulting from the presence of rings vinyl. While the third peak at 327 nm (30581cm-1) indicates the n-π* type transition, and this is due to each of the azomethine groups of the Schiff base and the thiazole ring[20]. The nuclear magnetic resonance (NMR) spectroscopy was used to identify the placement of protons in the obtained compound (DEAP), 1H-NMR (DMSO-d6) Fig. 2c: showed δ:11.19 (S,1H,OH) phenol, 7.51-7.82 (M,2H,CH) thiazole ring, 6.90, 6.65, 6,92 ,7,26 (M,4H,CH) phenyl ring,7.61,7.69,7.93-7.95 (M,10H,CH) benzil, 7.3,7.79 (M,4H,CH) benzylidenimine, 2.46 (S, 3H, CH3) methyl group .13C-NMR (DMSO-d6) Fig. 2d: show,169.28ppm(C18), 195.39ppm(C28), 139.07ppm(C31), 130.07ppm(C7C14C1C5), 131.91ppm(C12C6C3), 129.97ppm(C24C20C11C13C4C15), 128.14ppm(C33), 112.92ppm (C26), 106.95ppm (C36), 163.7ppm (C22), 154.09ppm (C9C10) ,139.07ppm (C32) ,26.32ppm (C17) [21-27].
The molecular weight of the Schiff base, as examined in DMSO solvent, was verified through mass spectral analysis [28, 29]. The mass spectra illustrated a parent peak at m/z 500.62 for the [M+H] ion of the compound (DEAP) as depicted in Fig. 3, aligning with the anticipated molecular weight, thereby validating the synthesis of DEAP.
X-ray diffraction elucidates the molecular or atomic arrangement in solid-state materials. Compound (DEAP)’s X-ray diffractograms were computed, and the intensity of diffracted CuKα radiation was measured in 2Ѳ between 5° and 80° (λ = 1.54060 Å; generator settings 30 mA/40 kV) [30, 31]. The Xpert High Score software calculated diffraction spectra parameters using identified diffraction peaks, revealing the compound’s crystalline nature (Fig. 4a). Major relaxes and corresponding d-spacing values were calculated using Bragg’s equation (nλ = 2dsinѲ), with d-spacing of 7.05A° at 2Ѳ = 12.55. The Debye-Scherrer equation (D = kλ/βCosѲ) calculated average crystallite size and size distributions, resulting in an average size of 39.07nm for DEAP. This suggests nanostructure properties due to the crystallite size. Field Emission Scanning Electron Microscopy (FE-SEM) revealed surface morphology, particle shape, aggregation, and distribution in compound (DEAP). The EDX spectrum (Fig. 4b) confirmed the presence of C, O, S, and N elements. The technique operated at a cross-sectional distance of 200nm and magnification force of 20.00 KX. The FE-SEM images (Fig. 4c) showed spherical crystals with an average particle size of 25.98nm, less than 100nm, indicating a nanoscale range. The increased surface area may facilitate the creation of new energy levels, enabling more free electron movement. The compound’s characteristics suggest potential use in medical applications, particularly in inhibiting various types of cancers[32-35].

Biological Activities
Thiazole core is integral to several clinically used anticancer drugs including dasatinib, dabrafenib, ixabepilone, patellamide A, and epothilone. Thiazole compounds demonstrate substantial efficacy in inhibiting various types of bacteria and fungi. Their effectiveness can be attributed to their ability to dissolve the outer cell wall, causing fluid loss and eventual cell death. The hybrid atoms, nitrogen and sulfur, bind to specific elements within the cell, such as copper, cobalt, iron, zinc, monovalent manganese, and monovalent potassium ions, which are essential for the bacterial cell. This interaction forms complexes with these elements, leading to cell death due to the depletion of these components [36, 37].

Minimum inhibition concentration )MIC)
Culture media
Prepared according to the manufacturing company instructions were autoclaved at 121°C for 15 minutes at 15 pounds per square inch (Psi).
a- Nutrient broth: used for bacterial activation, a single colony from old culture bacteria add to 3ml of nutrient broth after that, there were incubated for 24h at 37°C.
b- Mueller-Hinton broth: used to activate bacteria and make different compound concentrations. 
Resazurin: (Alamar Blue) solution was prepared by dissolving 0.015 g of resazurin in 100 ml sterile distilled water, a vortex mixer was used until well dissolved and stored at 4°C for a maximum of one week after preparation.
Compound (DEAP) was prepared at double serial dilutions (128-1024 μg/ml) from a 10mg/ml stock solution in a microliter plate, utilizing Mueller-Hinton broth as a diluent. All wells were inoculated with a 20μL bacterial suspension equivalent to McFarland standard no.0.5 (1.5×108 cfu/mL), excluding the negative control wells. The microliter plates were incubated at 37°C for a period of 18-20 hrs. Post incubation, 20 µL of resazurin dye was added to all wells, and a further incubation for 2 hours was conducted to observe any color transitions. The sub-MIC concentrations were visually determined in broth micro dilutions as the lowest concentrations exhibiting a color shift from blue to pink in the resazurin broth assay [38, 39].

Determination of compound (DEAP) MIC 
The broth microdilution method was used to determine the MIC of the compound (DEAP) in a 96-well microtiter plate. The susceptibility of the five isolates, Staphylococcus aureus, streptococcus mutans, pseudomonas aeruginosa, E. coli, and candida albican were tested by determining the MIC using a microtiter plate. The results showed that the concentration range from (256-512 µg/ml) to compound was the MIC which can inhibit the growth of Staphylococcus aureus, while the MIC of streptococcus mutans range between (512-1024 µg/ml) this for gram-positive bacteria. The results showed that the MIC of E. coli ranged from (256-512µg/ml) and for pseudomonas aeruginosa multidrug resistance the MIC ranged between (512-256 µg/ml). finally, The MIC of candida albican ranged from (256-512 µg/m) the MIC for a compound to different pathogenic bacteria was shown in Table 2 and Fig. 5.

Antibacterial activity of the compound (DEAP) 
The agar well diffusion method was used to detect the antibacterial activity of compound R against four isolates of pathogenic bacteria and one fungal at the MIC concentration according to Lewus [40].
1- Each bacterial isolate under study was grown in nutrient broth and incubated at 37 ºC for 18-24 hrs.
2- After the incubation period, a volume of 0.1 ml of each bacterial suspension was spread on the surface of nutrient agar at 37ºC for 24 hrs.
3- Single colony was added into a test tube having 5 mL of normal saline to yield a bacterial suspension of modest turbidity likened to the standard turbidity solution; this nearly equals 1.5x108 cfu/mL
4- Using a sterile cotton swab, a part of the bacterial suspension was transported cautiously and regularly spread on Mueller- Hinton agar medium then left for 10 min.
5- Five millimeter in diameter wells were made in the previous agar layer (3 wells per plate). The agar discs were removed, 50 µL of compound (DEAP) was added to each well using a micropipette, and the D.W was added to the middle well as a control. Plates were incubated at 37 ºC for 18 hrs. and after that, the diameter of inhibition zones was recorded. Table 3 showed the antibacterial activity assay of the compound (DEAP) at the MIC concentration of each isolate. The results indicated that DEAP compound has higher antibacterial activity against all pathogenic isolates. The difference in the biological activity of the compound (DEAP) is attributed to the resistance and sensitivity of bacterial isolates. Second, the difficulty and ease of dispersal of compounds in the culture medium, and finally, the solubility of the compound (DEAP) [41].

Cytotoxic activity
The Freshney method was utilized to cultivate both prostate and breast cancer cell lines. The cells were dissolved using a 37°C water bath, subsequently being positioned in a 25 cm^2 animal cell culture dish, containing RBMI-1640 culture medium and 10% calf serum. The culture dishes were incubated in a 5% carbon dioxide environment at 37°C for 24 hours. Post incubation, secondary cultures were created upon confirming uncontaminated cellular growth. The viability and contamination-free status of all cells were ensured via examination with an inverted microscope, confirming they had proliferated to the required number, approximately 1000 cells/ml. The prepared cells were relocated to a structure referred to as the growth cabin. The used culture medium was disposed of and the cells were cleansed with Physiological Saline Solution (PBS), a procedure repeated twice, each iteration lasting 10 minutes, after that, a sufficient amount of the enzyme trypsin was added to these cells and incubated for 30-60 seconds at a temperature of 37 °C. They were placed under observation until they changed from monolayer to single cells. At that time, the enzyme was stopped by adding a new growth medium containing blood serum cow-calf, after that, these cells were collected in the tubes of the centrifuge and placed in the device at a speed of 2000 rpm for ten minutes at room temperature, to precipitate the cells and get rid of trypsin, and the culture medium used. The filtrate was discarded and the cells were suspended in a new culture medium containing 10% of Calf serum. The filtrate was discarded and the cells were suspended in a new culture medium containing 10% of calf blood serum. After that, many cells were examined by taking a calculated and specific volume of the cell suspension plus the same volume of dye (Trypan Blue) to know the total number of cells and the percentage Vital using a slice (Hemocytometer) and according to the equation below: 

C = N x 104 x F/ml                                                 

Where C is the number of cells present in 1mL of the solution, N is the number of cells on the slide, F is the mitigation factor, and 104 is the slide’s dimensions. Then the vital percentage of cells in the sample was calculated using slide (Hemocytometer) according to the equation shown below, and the cell suspension was distributed in new containers and incubated in a 5% incubator of (CO2) at a temperature of 37°C, for (24) hours.

Vitality percentage of living cells = live cells/dead cells x 100 

Test the Dye of MTT To Examine the Vitality of Cells Principle of Testing
Test principle
The MTT method is used to detect the biological activity of anti-cancer compounds, through which the extent of the toxicity of chemicals on cells outside the body of the organism is revealed through electronic transmission of the plasma membrane, and to determine the effect of these substances and how to use them as a treatment if their toxicity towards infected cells is shown, provided that they are not effective on average, uninfected cells.
Tetrazolium salts are considered one of the indicators for measuring the level of activity of cells in prokaryotic organisms as well as eukaryotic organisms, assays are usually performed 3-(4,5 Dimethyl thiazol- 2-yl)-2,5-Diphenyl Tetrazolium Bromide (MTT)in the dark because the MTT detector is light sensitive, Where the percentage of live cells can be calculated through a colorimetric assay to measure the activity of cellular enzymes that reduce Tetrazolium dye in MTT after its conversion to blue-purple formazan is insoluble after the MTT dye changes color from yellow and is converted to blue-purple, as a result, increasing the intensity of the blue color means getting the largest number of live cells.
The color change is due to the production of an enzyme called dehydrogenase by the mitochondria, which is responsible for breaking the tetrazolium ring of the MTT dye. The anti-cancer activity of the compound (DEAP) was conducted by cell cytotoxicity and screening for in vitro antitumor activity against prostate cancer line PC3 and breast cancer (MCF-7) cell lines were evaluated by determining their cellular viability using the colorimetric (MTT) assay.

The Method of Work
Cancer line cells were processed by following the same steps described in paragraphs (2-18-1); then, the cellular suspension was placed in a plate containing 96 holes with a flat base, and it was incubated in an incubator with CO2 5% at a temperature of 37 °C for a full day (24 hours). After that, 100 microliters of that suspension were added in each hole, then the prepared concentrations of the compound under study were added ( 12.50, 25, 50, 100, 200, and 400 µg/ml) to the pits, at the rate of (3) pits for each concentration, after which the plate was incubated for 24 hours, i.e., a full day, and the incubation temperature was 37°C, and 10 microliters of MTT solution was added to each well, at a concentration of 0.45 mg/ml. Then the plate was incubated again for 4 hours at a temperature of 37°C; after that, 100 µL of solubilization solution was added to dissolve the Formazan Crystal solution. Finally, the absorbance of that sample was read at wavelength 570 nm using an ELASIS device.
Chemotherapy is the primary method for treating metastasized cancers. The compound (DEAP) have been screened for its anticancer property against breast cancer (MCF-7) cell lines and prostate cancer (PC3) using five different concentrations, and their effect on the normal cells (control) was also checked and operated by (MTT) protocol [42, 43]. Table 4, and Fig. 6 shows the effect of the compound (DEAP) on the growth process of prostate cancer cells (PC3) and normal healthy cells (HdFn). At a concentration of 400 µg/ml for cells of prostate cancer cell lines (PC3) as well as normal cells (HdFn), healthy cells were used to compare with prostate cancer cells (PC3) to find out the possibility of using them as a drug. The reactivity with the organic compound ranged between (96.80% - 71.95%) for PC3 prostate cancer cells, while the percentage ranged between (96.95% - 81.70%) for standard (HdFn) cells. It was found that the best inhibition rate for PC3 prostate cancer cells at The concentration of 400 µg / ml was 28.05%, while the percentage of inhibition of the cell line of healthy cells (HdFn) at the same concentration was 18.3%, which is almost a good result, as we need some modifications to increase this percentage. Among the important things that must be mentioned and that we have reached through the tests that were conducted for the organic compound and among the cells of prostate cancer lines (PC3 and normal cells ((HdFn) is (IC50) the half inhibitory concentration (Inhibition Concentration Fifty), as the half inhibitory concentration reached Fifty (IC50) through the interaction of the organic compound with the prostate cancer cell line (PC3) 161.2 µg/ml, while the half inhibitory concentration of healthy cells (HdFn) was 201.6 µg/mL represents the relationship between the biological activity of the prostate cancer cell line PC3 and cells of the normal cell line HdFn against the concentration of the organic compound. While the breast cancer (MCF-7) cell lines, It was noted that the inhibition percentages for the compound (DEAP) differed according to the type of cell line, as the number of live cells remaining after interaction with the organic compound ranged between (94.48% - 50.39%) for the cells of the breast cancer cell line (MCF-7), and between (94.83% - 71.57%) for cells of the normal cell line (HdFn). The highest percentage of inhibition of the compound (DEAP) appeared in the breast cancer cell line (MCF-7) at a concentration of 400 µg/ml, as the percentage of inhibition after reaction with the organic compound ranged 49.61%, while the highest percentage of inhibition appeared for the compound (DEAP) after The reactance of the normal cell line (HdFn) at the same concentration was 28.43% [29-31].

CONCLUSION
This study documents the synthesis and spectral characterization of a novel Schiff base compound, 2-(((1E,2E)-1,2-diphenyl-2-((4(€-1-(thiazol 2ylimino) ethyl) phenyl) imino) ethylidene) amino) phenol (DEAP), originating from thiazole, the structure of which is depicted in Fig. 7. The formation of DEAP was validated through FTIR, 1H 13C-NMR, and electronic spectral analysis. Various morphologies of the Schiff base compound were observed via XRD and FE-SEM. Additionally, DEAP exhibited significant biological activity against bacteria, along with selective cytotoxicity, damaging cancerous cell lines without harming normal cells, a property pivotal to future cytotoxic therapies. Findings suggested that such compounds might significantly inhibit the growth of cancer cells, positioning them as promising candidates for further structural modifications and pharmacological assessments. The potential for their application as anti-cancer drugs in medical and pharmaceutical fields is undeniable.

CONFLICT OF INTEREST
The authors declare that there is no conflict of interests regarding the publication of this manuscript.